simple pci imaging software  (Hamamatsu)

Journal: Fertility and sterility

Article Title: Sildenafil stimulates human trophoblast invasion through nitric oxide and guanosine 3′,5′-cyclic monophosphate signaling

doi: 10.1016/j.fertnstert.2015.02.025

Figure Lengend Snippet: Trophoblast invasion from first-trimester chorionic villous explants. First-trimester villous explants were cultured on Matrigel-coated transwell inserts and treated with vehicle or 350 ng/mL sildenafil for 72 hours before fixation. Examples of outgrowths from villous explants cultured with vehicle (A, C, E) or 350 ng/mL sildenafil (B, E, F) are shown. Bright-field images (A, B) and paired fields labeled with 6-diamino-2-phenylindole (C, D) or anti-Ki67 (E, F) are included. Size bar in (A) applies to all image panels. Outgrowth length and cell proliferation were measured using Simple PCI image analysis software (G, H). The graph in (G) shows outgrowth lengths for triplicate experiments in which 10 villous explants were measured for each treatment. * P<.05 vs. vehicle. The graph in (H) shows the percentage of cell nuclei in the outgrowth regions that were labeled with anti-Ki67 antibody. The differences between vehicle- and sildenafil-treated explants were not significant (n = 8). Error bars represent SEM.

Article Snippet: Images were processed to determine gray level using Simple PCI (Hamamatsu) imaging software, as previously described ( 31 , 32 ).

Techniques: Cell Culture, Labeling, Software

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Analysis of Vascular Hydrogen Sulfide Biosynthesis

doi: 10.1007/978-1-4939-9528-8_3

Figure Lengend Snippet: Semiquantitative assessment and localization of CBS and CSE. UA sections were labeled with antibodies against CBS, or CSE (not shown), followed by secondary Alexa488 (green)-labeled secondary antibody. Endothelial cells were labeled with CD31 followed by Alexa568 (red)-labeled secondary antibody. Cell nuclei were stained with DAPI. Negative control treated with IgG or with primary antibody omitted is shown in first panel. Representative outline of border between UA intima and media was indicated, and UA lumen (l), intima (i), and media (m) were denoted (second panel). Regions of interest for intima (ROI – i) and media (ROI – m) used for analysis in Simple PCI software are illustrated (third panel). Scale bar is 25 μm

Article Snippet: Image Analysis list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Open image in Simple PCI Image analysis software (Hamamatsu Corp, Sewickley, PA, USA).

Techniques: Labeling, Staining, Negative Control, Software